Associate Professor Benjamin M. Hogan
Principal Investigator | Hogan Lab
Co-Director UQ Centre for Cardiac and Vascular Biology
NHMRC & Heart Foundation Career Development Fellow
Co-Division Head Genomics Of Development and Disease, Institute for Molecular Bioscience, the University of Queensland
Affiliated Senior Research Fellow SBMS, Faculty Of Medicine And Biomedical Sciences, the University of Queensland
I completed my PhD in 2004 followed by a period as a Cancer Council postdoctoral fellow at the Ludwig Institute (Melbourne) using the zebrafish model. In 2006, I moved to the Hubrecht Institute in the Netherlands where I applied large-scale zebrafish forward genetic screening to study lymphatic vascular development in the embryo. In the first vertebrate mutagenesis screen for lymphatic mutants, several new molecular regulators of lymphatic development were identified, including Ccbe1, later found to be responsible for lymphatic dysplasia and lymphoedema in Hennekam syndrome patients. In 2010, I returned to Australia as a group leader and ARC Future Fellow at the Institute for Molecular Bioscience, Brisbane. I remain focused on understanding the process of vascular development in the vertebrate embryo with a focus on lymphatics.
Lymphatic vessels are used by tumours to spread throughout the body, they also play crucial roles in common cardiovascular diseases. Hence, there is a significant need to be able to either restrict of promote lymphatic vessel formation in disease. By gaining a deep understanding of the molecular mechanisms that control vessel development, my laboratory hopes to open up new molecular approaches to manipulate vessel growth.
Our research investigates how our vascular lineages and networks form during the development of the vertebrate embryo. We aim to discover new genes and molecular pathways essential for vessel development, primarily using the zebrafish embryo as a model biological system. We couple whole genome sequencing with phenotype based screening and genome editing to study gene function and mechanism. We also image vascular development with ever-increasing experimental resolution to observe and understand novel developmental processes for the first time.
Overman J, Fontaine F, Moustaqil M, Mittal D, Sierecki E, Sacilotto N, Zuegg J, Robertson AAB, Holmes K, Salim AA, Mamidyala S, Butler MS, Robinson AS, Johnston W, Alexandrov K, Black BL, Hogan BM, De Val S, Capon RJ, Carroll JS, Bailey TL, Koopman P, Jauch R, Smyth MJ, Cooper MA, Gambin Y, Francois M. Pharmacological targeting of the transcription factor SOX18 delays breast cancer in mice. Elife 2017 Jan 31;6. pii: e21221. Pubmed
De Angelis JE*, Lagendijk AK*, Chen H, Tromp A, Bower NI, Tunny KA, Brooks AJ, Bakkers J, Francois M, Yap AS, Wicking C, Hogan BM#, Smith KA#. Tmem2 regulates embryonic Vegf-signaling by controlling Hyaluronic acid turnover. Developmental Cell 2017. Jan 23 Volume 40, Issue 2, p123–136. *Joint first authors #Joint senior authors. Pubmed
Bower NI, Vogrin A, Le Guen L, Chen J, Stacker SA, Achen MG, Hogan BM. Vegfd modulates both angiogenesis and lymphangiogenesis during zebrafish embryonic development. Development 2017 Jan 13;144:507-518. Pubmed
Capon SJ, Baillie GJ, Bower NI, da Silva JA, Paterson S, Hogan BM, Simons C, Smith KA. Utilising polymorphisms to achieve allele-specific genome editing in zebrafish. Biology Open 2017 Jan 15;6(1):125-131. Pubmed
Crawford J, Bower NI, Hogan BM, Taft RJ, Gabbett MT, McGaughran J, Simons C. Expanding the genotypic spectrum of mutations in Hennekam syndrome. Am J Med Genet A 2016 Oct;170(10):2694-7. Pubmed
Koenig AL, Baltrunaite K, Bower NI, Rossi A, Stainier DY, Hogan BM, Sumanas S. Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein. Developmental Biology 2016 Mar 1; 411(1):115-27. Pubmed
Koltowska K*, Lagendijk AK*, Pichol-Thievend C, Fischer JC, Francois M, Ober EA, Yap AS, Hogan BM. Vegfc Regulates Bipotential Precursor Division and Prox1 Expression to Promote Lymphatic Identity in Zebrafish. Cell Reports 2015 Dec 1; 13(9): 1828–1841. *Co-first authors. Journal cover art
Koltowska K, Paterson S, Bower NI, Baillie GJ, Lagendijk AK, Astin JW, Chen H, Francois M, Crosier PS, Taft RJ, Simons C, Smith KA, Hogan BM. mafba is a downstream transcriptional effector of Vegfc signaling essential for embryonic lymphangiogenesis in zebrafish. Genes & Development 2015 Aug 1;29(15):1618-30. Cover art
Jeffery J, Neyt C, Moore W, Paterson S, Bower NI, Chenevix-Trench G, Verkade H, Hogan BM, Khanna KK. Cep55 regulates embryonic growth and development by promoting Akt stability in zebrafish. The FASEB Journal 2015 May; 29(5): 1999-2009.
Wu SK, Lagendijk AK, Hogan BM, Gomez GA, Yap AS. Active contractility at E-cadherin junctions and its implications for cell extrusion in cancer. Cell Cycle. 2015 Feb; 14(3):315-22.
Coxam B, Neyt C, Grassini DR, Le Guen L, Smith KA, Schulte-Merker S, Hogan BM. carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish. Developmental Dynamics. 2015 Jan; 244(1):1-9.
Astin JW, Haggerty MJ, Okuda KS, Le Guen L, Misa JP, Tromp A, Hogan BM, Crosier KE, Crosier PS. Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting. Development. 2014 Jul; 141(13):2680-90.
Chang GH, Lay AJ, Ting KK, Zhao Y, Coleman PR, Powter EE, Formaz-Preston A, Jolly CJ, Bower NI, Hogan BM, Rinkwitz S, Becker TS, Vadas MA, Gamble JR. ARHGAP18: an endogenous inhibitor of angiogenesis, limiting tip formation and stabilizing junctions. Small GTPases. 2014 Jul; 5(3):1-15.
Coxam B, Sabine A, Bower NI, Smith KA, Skoczylas R, Astin J, Crosier P, Parton R, Harvey N, Petrova T, Schulte-Merker S, Francois M, Hogan BM. Pkd1 regulates lymphatic vascular morphogenesis during development. Cell Reports. 2014. 2014 May 8; 7(3):623-33.
Le Guen L, Karpanen T, Schulte D, Harris N, Koltowska K, Roukens G, Bower NI, Van Impel A, Stacker SA, Achen MG, Schulte-Merker S, Hogan BM. Ccbe1 regulates Vegfc-mediated induction of Vegfr3 signaling during embryonic lymphangiogenesis. Development. 2014 Mar; 141(6):1239-49.
Kartopawiro J, Bower NI, Karnezis T, Kazenwadel J, Betterman KL, Lesieur E, Koltowska K, Astin J, Crosier P, Vermeren S, Achen MG, Stacker SA, Smith KA, Harvey NL, Francois M, Hogan BM. Arap3 is dysregulated in a mouse model of hypotrichosis- lymphedema-telangiectasia and regulates lymphatic vascular development. Human Molecular Genetics 2014 Mar 1; 23(5):1286-97.
Duong T, Koltowska K, Pichol-Thievend C, Le Guen L, Fontaine F, Smith KA, Truong V, Skoczylas R, Stacker SA, Achen MG, Koopman P, Hogan BM*, Francois M* VEGFD regulates blood vascular development by modulating SOX18 activity. Blood. 2014 Feb; 123(7):1102-12. (*Equal last author) Journal Cover Art
Keightley, M. C.; Crowhurst, M. O.; Layton, J. E.; Beilharz, T.; Markmiller, S.; Varma, S.; Hogan, B. M.; de Jong-Curtain, T. A.; Heath, J. K.; Lieschke, G. J. In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation FEBS Lett. 2013 July; 587(14):2150-7 .
Cermenati S, Moleri S, Neyt C, Bresciani E, Carra S, Grassini DR, Omini A, Goi M, Cotelli F, François M, Hogan BM, Beltrame M. Sox18 Genetically Interacts With VegfC to Regulate Lymphangiogenesis in Zebrafish. Arterioscler Thromb Vasc Biol. 2013 Jun; 33(6):1238-47.
Macheda ML, Sun WW, Kugathasan K, Hogan BM, Bower NI, Halford MM, Zhang YF, Jacques BE, Lieschke GJ, Dabdoub A, Stacker SA. The Wnt Receptor Ryk Plays a Role in Mammalian Planar cell Polarity Signaling. J Biol Chem. 2012 Jul 6.
Tao S, Witte M, Bryson-Richardson R, Currie PD, Hogan BM*, Schulte-Merker S*. Zebrafish prox1b mutants develop a lymphatic vasculature and prox1b does not specifically mark lymphatic endothelial cells. PLoS One. 2011. *Equal last author
Smith KA, Lagendijk AK, Courtney AD, Chen H, Paterson S, Hogan BM, Wicking C, Bakkers J. Transmembrane protein 2 (Tmem2) is required to regionally restrict atrioventricular canal boundary and endocardial cushion development. Development. 2011 Oct;138(19):4193-8.
Geudens I, Herpers R, Hermans K, Segura I, Ruiz de Almodovar C, Bussmann J, De Smet F, Vandevelde W, Hogan BM, Siekmann A, Claes F, Moore JC, Pistocchi AS, Loges S, Mazzone M, Mariggi G, Bruyère F, Cotelli F, Kerjaschki D, Noël A, Foidart JM, Gerhardt H, Ny A, Langenberg T, Lawson ND, Duckers HJ, Schulte-Merker S, Carmeliet P, Dewerchin M. Role of delta-like 4/Notch in the formation and wiring of the lymphatic network in zebrafish. Arterioscler Thromb Vasc Biol. 2010 Sep;30(9):1695-702
Alders M, Hogan BM, Gjini E, Salehi F, Al-Gazali L, Hennekam EA, Holmberg EE, Mannens MM, Mulder MF, Offerhaus GJ, Prescott TE, Schroor EJ, Verheij JB, Witte M, Zwijnenburg PJ, Vikkula M, Schulte-Merker S, Hennekam RC. Mutations in CCBE1 cause generalized lymph vessel dysplasia in humans. Nature Genetics. 2009 Dec;41(12):1272-4.
Hogan BM, Herpers R, Witte M, Heloterä H, Alitalo K, Duckers HJ, Schulte-Merker S. Vegfc/Flt4 signalling is suppressed by Dll4 in developing zebrafish intersegmental arteries. Development. 2009 Dec; 136(23):4001-9.
Voss K, Stahl S, Hogan BM, Reinders J, Schleider E, Schulte-Merker S, Felbor U. Functional analyses of human and zebrafish 18 amino acid in-frame deletion pave the way for domain mapping of the cerebral cavernous malformation 3 (CCM3) protein. Human Mutation. 2009 Jun; 30(6):1003-11.
Hogan BM, Bos F, Witte M, Bussmann J, Dukkers H, Schulte-Merker S. Ccbe1 is required for embryonic lymphangiogenesis and venous sprouting. Nature Genetics. 2009. Apr;41(4):396-8.
Hogan BM, Bussmann J, Wolburg H, Schulte-Merker S. ccm1 cell autonomously regulates endothelial cellular morphogenesis and vascular tubulogenesis in zebrafish. Human Molecular Genetics. 2008 Aug 15;17(16):2424-32.
Dworkin S, Heath JK, deJong-Curtain TA, Hogan BM, Lieschke GJ, Malaterre J, Ramsay RG, Mantamadiotis T. CREB activity modulates neural cell proliferation, midbrain-hindbrain organization and patterning in zebrafish. Developmental Biology. 2007 Jul 1;307(1):127-41.
Hogan BM, Pase L, Hall NE, Lieschke GJ. Characterisation of duplicate zinc finger like 2 erythroid precursor genes in zebrafish. Development Genes and Evolution. 2006 Sep;216(9):523-9.
Hogan BM, Layton JE, Pyati UJ, Nutt SL, Hayman JW, Varma S, Heath JK, Kimelman D, Lieschke GJ. Specification of the primitive myeloid precursor pool requires signaling through Alk8 in zebrafish. Current Biology. 2006 Mar 7;16(5):506-11.
Hogan BM, Danks JA, Layton JE, Hall NE, Heath JK, Lieschke GJ. Duplicate zebrafish pth genes are expressed along the lateral line and central nervous system during embryogenesis. Endocrinology. 2005 Feb;146(2):547-51.
Hogan BM, Hunter MP, Oates AC, Crowhurst MO, Hall NE, Heath JK, Prince VE, Lieschke GJ. Zebrafish gcm2 is required for gill filament budding from pharyngeal ectoderm. Developmental Biology. 2004 Dec 15;276(2):508-22.
Mawdsley DJ, Cooper HM, Hogan BM, Cody SH, Lieschke GJ, Heath JK. The Netrin receptor Neogenin is required for neural tube formation and somitogenesis in zebrafish. Developmental Biology. 2004 May 1;269(1):302-15.
Behan KJ, Nichols CD, Cheung TL, Farlow A, Hogan BM, Batterham P, Pollock JA. Yan regulates Lozenge during Drosophila eye development. Development Genes and Evolution. 2002 Jul;212(6):267-76.
Reviews and Book Chapters
Pichol-Thievend C, Hogan BM, Francois M. Lymphatic vascular specification and its modulation during embryonic development. Microvascular Research. 2014 Nov; 96:3-9. (Special Issue: Lymphatics in Development and Pathology) Review
Lagendijk AK, Yap A, Hogan BM. Endothelial cell-cell adhesion during zebrafish vascular development. Cell adhesion and migration. 2014 Feb; 8(2):136-45. Review
Koltowska K, Betterman KL, Harvey NL, Hogan BM. Getting out and about: the emergence and morphogenesis of the vertebrate lymphatic vasculature. Development. 2013 May; 140(9):1857-70. Review
Francois M, Harvey NL, Hogan BM. The transcriptional control of lymphatic vascular development. Physiology. 2011 Jun; 26(3):146-55. Review
Lagendijk AK and Hogan BM. VE-Cadherin in vascular development: a coordinator of cell signalling and tissue morphogenesis. Current Topics in Developmental Biology 2015; 11. In press. Book Chapter
Soroldoni D, Hogan BM, Oates AC. Simple, efficient transient and stable transgenesis with meganuclease constructs in zebrafish. Methods in Molecular Biology. 2009; 546:117-30. Book Chapter
Hogan BM and Schulte-Merker S. New animal models of lymphangiogenesis. (Book Series: Cancer Metastasis Biology and Treatment). Ed: Stacker S and Achen MG. Lymphangiogenesis in cancer metastasis. Springer. 2009; 13:27-54. Book Chapter
Hogan BM, Verkade H, Lieschke GJ, Heath JK. Manipulation of gene expression during zebrafish embryonic development using transient approaches. Methods in Molecular Biology. 2008; 469:273-300. Book Chapter
2016, ANZSCDB Emerging Leader Award, Australia and New Zealand Society for Developmental Biology
2015, Recipient of Heart Foundation Future Leader Fellowship*, National Heart Foundation
2015, Recipient of NHMRC Career Development Fellowship and National Health and Medical Research Council Australia Fellowship (Co-funded)
2015, Researcher of the Year Award for 2014, National Heart Foundation
2011, Recipient of ARC Future Fellowship, Australian Research Council
2010, Foundation Research Excellence Award, University of Queensland
2009, Postdoctoral Investigators Award, National Association of Research Fellows (NARF) of the NHMRC Australia
2007, Recipient of EMBO Postdoctoral Fellowship, European Molecular Biology Organization
2007, Recipient CJ Martin Postdoctoral Fellowship, National Health and Medical Research Council Australia
2005, Recipient of Cancer Council of Victoria Postdoctoral Fellowship, Cancer Council of Victoria
2004, Ludwig Institute Student Medal for achievements in PhD research, Ludwig Institute for Cancer Research, Melbourne
2001-2004 Doctor of Philosphy, University of Melbourne, The Ludwig Institute for Cancer Research
2000-2000 Honours 1st Class, University of Melbourne
1996-1999 Bachelor of Science, University of Melbourne, University of Glasgow