Welcome to the
Muscle Tissue Engineering Laboratory


 

Hudson Porello laboratory heart tissue

 

Hudson Laboratory

Our laboratory is interested in understanding the molecular mechanisms and biological interactions that guide the formation, properties and function of human cardiac organoids. We are particularly focussed on the mechanisms driving endogenous regenerative capacity of cardiac muscle and have recently discovered an innate regenerative capacity in immature cardiac organoids similar to that recently reported in newborn mammals. We have now developed miniaturised screening platforms for cardiac organoids and hope not only to discover new targets for regenerative medicine, but also use these platforms to develop regenerative therapeutics.


The Muscle Tissue Engineering Laboratory, led by James Hudson, is located at the School for Biomedical Sciences. Our laboratory uses state-of-the-art bioengineering techniques to produce functional human cardiac organoids. We are using these organoids to discover the fundamentals of development, multi-cellular interactions and function.
 

The Hudson Lab

  • 5

    Researchers

  • 22

    Publications

  • 4

    Projects

  • 389

    Citations

The Hudson Laboratory research


Cardiovascular disease is the leading cause of death worldwide. Due to the very limited regenerative capacity of the adult mammalian heart, diseases that result in cardiomyocyte death, such as myocardial infarction, can have devastating consequences for organ function. In contrast, the mammalian heart harbours a transient potential for regeneration during neonatal life, which is lost shortly after birth. Similar to the zebrafish and axolotl, the neonatal mouse heart can mount a robust regenerative response following multiple types of cardiac injury (surgical amputation, cryoinjury and myocardial infarction). This regenerative capacity appears to be primarily driven by the proliferation of resident cardiomyocytes. However, the molecular mechanisms that regulate cardiomyocyte proliferative potential and cardiac regenerative capacity are poorly understood.
 

Porello Hudson heart research heart immunoflourescence


Our laboratory at The University of Queensland in Brisbane, Australia, is trying to unravel the molecular mechanisms that drive endogenous cardiac regeneration and maturation. Some of the projects currently being pursued in our laboratory include:

 

Modelling regeneration using human cardiac organoids.

Recent advances in stem cell sciences and tissue engineering are providing unparalleled opportunities to generate human heart tissues in a dish. We are using a newly developed human cardiac organoid injury model to understand the mechanisms that regulate endogenous regeneration of human heart muscle.

 

Drivers of cardiac maturation.

Human pluripotent stem cell derived cardiomyocytes are typically immature. While tissue engineering can be used to promote unprecedented maturation, the cardiomyocytes in these tissues are still missing some attributes of adult cardiomyocytes in vivo. We are using human cardiac organoids to find the key drivers of cardiac maturation and the mechanisms behind the maturation process.

 

Maturation of cardiac gene regulatory networks.

Epigenetic modifications are critical for the precise temporal regulation of gene expression during heart development. We are trying to map the epigenetic changes that occur as cardiomyocyte mature and the mechanisms of how this shuts down cell cycle activity.

 

 

 

 

Regulation of endogenous heart regeneration by an anti-fibrotic microRNA

2015-2017, NHMRC Project Grant, Dr Enzo Porello, Dr James Hudson
 

Regulation of heart development and regeneration by DNA methylation

2015-2017, NHMRC Project Grant, Dr Enzo Porello, Dr James Hudson
 


RECENTLY COMPLETED PROJECTS

 

Development of new heart failure therapeutics by analysing signalling heart failure as a network

2014-2016, Early Career Fellowship, Peter Doherty Australian Biomedical Fellowship, NHMRC, Dr James Hudson

 

Discovery of novel heart failure therapeutics via development of a next generation therapeutic screening platform: Force-generating human heart tissue micro-arrays

2014-2016, NHMRC Project Grant, Dr James Hudson

 


CURRENT FELLOWSHIPS


Using human 3D engineered heart tissue for discovery of novel biology and novel therapeutics

2017-2020, RD Wright Biomedical Career Development Fellowship, NHMRC - Dr James Hudson
 

Using human 3D engineered heart tissue for discovery of novel biology and novel therapeutics

2017-2020, Future Leader Fellowship, Paul Korner Innovation Award, National Heart Foundation – Dr James Hudson


 

David A. Elliott
Cardiac Development Laboratory, Cell Biology Theme, Murdoch Childrens Research Institute, Melbourne, AUSTRALIA

Assam El-Osta
Baker IDI Heart and Diabetes Institute, Melboune, AUSTRALIA

Mat Francois
Division of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, AUSTRALIA

Paul Gregorevic
Baker IDI Heart and Diabetes Institute, Melboune, AUSTRALIA

Richard Harvey
Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, Sydney, AUSTRALIA

David James
Charles Perkins Centre (CPC), University of Sydney, Sydney, AUSTRALIA

Bradley Launikonis
School of Biomedical Sciences (SBMS), The University of Queensland, Brisbane, AUSTRALIA

Stuart Mazzone
School of Biomedical Sciences (SBMS), The University of Queensland, Brisbane, AUSTRALIA

Rob Parton
Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, Brisbane, AUSTRALIA

Kelly Smith
Division of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, AUSTRALIA

Walter Thomas
School of Biomedical Sciences (SBMS), The University of Queensland, Brisbane, AUSTRALIA

Qing-Dong Wang
Cardiovascular and Metabolic Diseases, Innovative Medicines and Early Development, AstraZeneca, SWEDEN

 

 

 
 

The Hudson Laboratory discoveries in the media

 
 TELEVISION, RADIO, NEWSPAPER & MAGAZINE REPORTS

 

17 Mar 2017 University of Queensland scientist have created a beating human heart muscle
Two UQ School of Biomedical Sciences researchers, Dr James Hudson and Dr Enzo Porrello, and German colleagues have created a 'beating' human heart muscle from stem cells. The discovery and interviews with Dr James Hudson and Rachelle Foreman from the Queensland Heart Foundation aired on Australian television news - Seven News (Brisbane, Bundaberg), ABC News (Hobart, Adelaide, Perth), and ABC News 24 National (Sydney, Melbourne, Regional NSW, Brisbane, Adelaide, Perth, Regional Queensland, Hobart, Canberra, Regional Victoria, Regional West Australia).

The story was also broadcast on radio - ABC Radio (Capricornia, Brisbane, Adelaide, Perth), Radio National (Canberra, Sydney, Melbourne, Brisbane, Perth, Hobart, Adelaide, Darwin, Newcastle) and featured in the Courier-Mail newspaper, Brisbane.

 


Seven News A world-first research at the University of Queensland aims to find a way to repair damaged heart tissue using stem cells
Watch the Seven News story and interview with Dr James Hudson
 

 

 



ABC News 24 University of Queensland scientists have created a beating human heart muscle using stem cells, paving the way for heart regeneration in heart attack victims

ABC News Adelaide Scientists from the University of Queensland say they have made a breakthrough in cardiac research

 

 

 

Brisbane Courier-Mail Scientists create human heart muscle
Read the article

 

 

 

 

International Innovation Magazine logoInternational Innovation Magazine, Issue 166 ‘Limitless’, Healing Hearts
Interview with Dr James Hudson, on unravelling the molecular mechanisms driving cardiac regeneration.

Download PDF of James' interview

 

 

 

 

 

 ONLINE MEDIA REPORTS

 

17 Mar 2017, Scientists create 'beating' human heart for cardiac research

Two UQ School of Biomedical Sciences researchers, Dr James Hudson and Dr Enzo Porrello, and German colleagues have created a 'beating' human heart muscle from stem cells. The research is published in Circulation and Development. In addition to television and radio, the story featured on 7 online news sites including; ABC Online, Yahoo! News Australia, LabOnline, BioPortfolio, get STEM, Medical Xpress and Health Canal.

 

ABC Online Self-repairing heart tissue breakthrough brings hope for cardiac patients

Yahoo! News Australia UQ conducts research into stem cells repairing damaged heart tissue

BioPortfolio and LabOnline The tell-tale heart: 'beating' human heart muscle created from stem cells

get STEM, Medical Xpress, Health Canal Scientists create 'beating' human heart for cardiac research

 

 

15 Mar 2017, The Node, the Company of Biologists, The people behind the papers: Holly Voges, Enzo Porrello and James Hudson
Based on the Voges et al publication, "Development of a human cardiac organoid injury model reveals innate regenerative potential" in the special issue of Development devoted to organoids.

 

 



UQ NEWS and FACULTY OF MEDICINE NEWS

17 Mar 2017, Scientists create 'beating' human heart for cardiac research
Two UQ School of Biomedical Sciences researchers, Dr James Hudson and Dr Enzo Porrello, and German colleagues have created a 'beating' human heart muscle from stem cells. The story featured on UQ News and the Faculty of Medicine News, in addition to television, radio and numerous online scientific and news websites.

 

UQ News article

 

 

 

 

UQ scientists create ‘beating’ human heart muscle from The University of Queensland on Vimeo.

 

 

2015, SBMS researcher awarded UQ 2015 Early Career Researcher (ECR) Grant

Dr Hudson was awarded an ERC grant from UQ to assist with his on-going research into developing tissue heart cells with a stem cell population. The UQ scheme aims to encourage research by new staff members by supporting high quality research projects from early career researchers and by providing limited seed funding to generate external research support.

News article

 

 

 

 

 

The Hudson Laboratory publications

 
 
 

 

Kaakinen, M, Reichelt ME, Ma Z, Ferguson C, Martel N, Porrello ER, Hudson JE, Thomas WG, Parton RG, Headrick JP. Cavin-1 Deficiency Modifies Myocardial and Coronary Function, Stretch Responses and Ischaemic Tolerance: Roles of NOS Over-Activity. Basic Research in Cardiology Accepted for publication 2 March 2017.

 

Tiburcy M, Hudson JE, Balfanz P, Schlick S, Meyer T, Liao M-LC, Levent E, Raad F, Zeidler S, Wingender E, Riegler J, Wang M, Gold JD, Kehat I, Wettwer E, Ravens U, Dierickx P, van Laake LW, Goumans MJ, Khadjeh S, Toischer K, Hasenfuss G, Couture LA, Unger A, Linke WA, Araki T, Neel B, Keller G, Gepstein L, Wu JC, Zimmermann W-H. Defined Engineered Human Myocardium with Advanced Maturation for Applications in Heart Failure Modelling and Repair. Circulation In press, accepted 24 Jan 2017.

 

Voges HK, Mills RJ, Elliott DA, Parton RG, Porrello E, Hudson J. Development of a human cardiac organoid injury model reveals innate regenerative potential. Development. 2017 Mar 15;144(6):1118-1127.

 

Vivien CJ, Hudson JE*, Porrello ER*. Evolution, Comparative Biology and Ontogeny of Vertebrate Heart Regeneration. npj Regenerative Medicine. 2016 1: 16012.

 

Titmarsh D, Glass N, Mills R, Hidalgo A, Wolvetang E, Porrello E, Hudson J, Cooper-White J. Induction of Human iPSC-Derived Cardiomyocyte Proliferation Revealed by Combinatorial Screening in High Density Microbioreactor Arrays. Scientific Reports 2016 Apr 21;6:24637.

 

Sim CB, Ziemann M, Kaspi A, Harikrishnan KN, Ooi J, Khurana I, Chang L, Hudson JE, El-Osta A, Porrello ER. Dynamic changes in the cardiac methylome during postnatal development. FASEB Journal 2015 29(4):1329-1343.

 

Ovchinnikov DA, Hidalgo A, Yang S-K, Zhang X, Hudson J, Mazzone SB, Chen C, Cooper-White JJ, and Wolvetang EJ. Isolation of Contractile Cardiomyocytes from Human Pluripotent Stem-Cell-Derived Cardiomyogenic Cultures Using a Human NCX1-EGFP Reporter. Stem Cells Dev 2015 24(1):11-20.

 

Titmarsh DM, Hudson JE, Hidalgo A, Elefanty A, Stanley EG, Wolvetang EJ, Cooper-White JJ. Microbioreactor Arrays for Full Factorial Screening of Exogenous and Paracrine Factors in Human Embryonic Stem Cell Differentiation. PLoS ONE 2012 7(12): e52405.

 

Hudson JE, Titmarsh DM, Hildago A, Wolvetang EJ, Cooper-White JJ. Primitive Cardiac Cells from Human Embryonic Stem Cells. Stem Cells Dev 2012 10; 21(9):1513-23.

 

Frith JE, Mills RJ, Hudson JE, Cooper-White JJ. Tailored Integrin-Extracellular Matrix Interactions to Direct Human Mesenchymal Stem Cell Differentiation. Stem Cells and Development 2012 21(13):2442-56.

 

Hudson JE, Brooke G, Blair C, Wolvetang EJ, Cooper-White JJ. Development of Myocardial Constructs Using Modulus-Matched Acrylated Polypropylene Glycol Triol (aPPGT) Substrate and Different Non-Myocyte Cell Populations. Tissue Eng Part A 2011 17(17-18):2279-89.

 

Mills RJ, Frith JE, Hudson JE, Cooper-White JJ. Effect of geometric challenges on cell migration. Tissue Eng Part C 2011 17(10):999-1010.

 

Frith JE*, Hudson JE*, Cooper-White JJ. Tissue engineering and regenerative medicine international society - Asia Pacific (TERMIS-AP) meeting 2010. Regenerative Medicine. 2011;6:167-170.

 

Hudson JE, Mills RJ, Frith JE, Brooke G, Jaramillo-Ferrada P, Wolvetang EJ, Cooper-White JJ. A defined medium and substrate for expansion of human mesenchymal stromal cell progenitors that enriches for osteo- and chondro-genic precursors. Stem Cells Dev 2011 20(1):77-87.

 

Hudson JE, Frith JE, Donose BC, Rondeau E, Mills RJ, Wolvetang EJ, Brooke G, Cooper-White JJ. A synthetic elastomer based on acrylated polypropylene glycol triol with tunable modulus for tissue engineering applications. Biomaterials 2010;31:7937-7947.

 

Markway BD, Tan G-K, Brooke G, Hudson JE, Cooper-White JJ, Doran MR. Enhanced Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells in Low Oxygen Environment Micropellet Cultures. Cell Transplantation 2010 19(1):29-42.

 

 

 

 

 

Hudson JE*, Porrello ER*. Periostin Paves the Way for Neonatal Heart Regeneration. Cardiovascular Research In press, accepted 2 Feb 2017.

 

Quaife-Ryan GA, Sim CB, Porrello ER*, Hudson JE*. Resetting the epigenome for heart regeneration. Seminars in Cell and Developmental Biology. 2016 58:2-13.

 

Hudson JE, Porrello ER. The Non-Coding Road Towards Cardiac Regeneration. Journal of Cardiovascular Translational Research 2013 6(6):909-923.

 

Christalla P*, Hudson JE*, Zimmermann W-H. The Cardiogenic Niche as Fundamental Building Block of Engineered Myocardium. Cells Tissues Organs 2012 195(1-2):82-93. *Authors contributed equally

 

Hudson JE, Zimmermann W-H. Tuning Wnt-signaling to enhance cardiomyogenesis in human embryonic and induced pluripotent stem cells. Journal of Molecular and Cellular Cardiology 2011 51(3):277-9.

 

Rowlands A, Hudson JE, Cooper-White JJ. From scrawny to brawny: the quest for neomusculogenesis; smart surfaces and scaffolds for muscle tissue engineering. Expert Rev Med Devices. 2007;4:709-728.

 

 

 

1. Mills RM, Titmarsh DM, Porrello ER, Hudson JE. 2016. Cardiac Maturation. Provisional filing PAT-02238.

A screening device and conditions to promote maturation of human cardiac organoids.

 

2. Quaife-Ryan, GA, Hudson JE, Porrello ER. 2016, Cardiac regeneration. Provisional filing PAT-02255.

A therapy to induce cardiac regeneration.

 

3. Tiburcy M, Hudson JE, Zimmermann W.-H., 2013. Method for producing Engineered Heart Muscle (EHM) - EP13181352.9

Method for producing defined, serum-free, human cardiac organoids.

 

4. Hudson JE, Tiburcy M, Zimmermann W.-H., 2013. A method to direct differentiation of pluripotent stem cells into functional heart muscle - EP13185344.2

Method for directing differentiation to produce defined, serum-free, human cardiac organoids.